Journal: Bioactive Materials
Article Title: A bioactive material with dual integrin-targeting ligands regulates specific endogenous cell adhesion and promotes vascularized bone regeneration in adult and fetal bone defects
doi: 10.1016/j.bioactmat.2022.05.027
Figure Lengend Snippet: Adhesion of human BMSCs, osteoblasts, ECFCs and UVECs on the LLP2A/LXW7 modified biomaterials in vitro . a, Quantitation of total DNA of human BMSCs, osteoblasts, ECFCs and UVECs adhered biomaterials modified with different integrin binding ligands. b , Quantitation of total DNA of human BMSCs, osteoblasts, ECFCs and UVECs adhered on biomaterials modified with different integrin binding ligands after integrin α4β1 expressed on the cells was blocked. c , Quantitation of total DNA of human BMSCs, osteoblasts, ECFCs and UVECs adhered on biomaterials modified with different integrin binding ligands after integrin αvβ3 expressed on the cells was blocked. d , Quantitation of total DNA of human BMSCs, osteoblasts, ECFCs and UVECs adhered on biomaterials modified with different integrin binding ligands after integrins α4β1 and αvβ3 expressed on the cells were blocked. Data were expressed as mean ± standard deviation: **p < 0.01, ***p < 0.001 (n = 6).
Article Snippet: Human umbilical vein endothelial cells (UVECs) were purchased from ATCC (PCS-100-013) and expanded in Endothelial Cell Growth Medium-2 (EGM-2, CC-3162, Lonza).
Techniques: Modification, In Vitro, Quantitation Assay, Binding Assay, Standard Deviation